Bioreactor in Tissue Engineering

(Department)  Biomedical Engineering         (Division)      Bioelectric
 (Level and Major) 

Course Title                 Bioreactor in Tissue Engineering
Number of Credits       3             Prerequisite Same as Tissue Engineering Field

Course Description:
We are going to learn about the third elements for tissue engineering; bioreactor. You would learn how to use different area of science to mimic the microenvironment inside body for cells. This microenvironment includes the geometry of the environment, media for cell culture, the flow of media, and different signaling.
Course Goals and objectives:
At the end of this course, students should be able to:
  • Identify the key required parameters for their tissue of the interest (biophysics, biology, biochemistry, and biomechanics)
  • Gather the required data for the important parameters
  • Propose the initial characters of the required bioreactor
  • Identify the inputs and outputs
  • Simplify the bioreactor
Course Topics:
  • Bioreactor for tissue engineering; where different fields meet each other
  • A review on the basic concepts and definitions in chemical and biological reactions (reaction, reaction rate, reaction element, open system vs closed one)
  • Batch reactor
  • Continuous stirred reactor (CSTR)
  • Plug flow reactor (PFR) and packed bed reactor (PBR)
  • Conversion and design equations for batch and CSTR reactors
  • Conversion and design equations for PFR and PBR
  • Series reactors vs parallel reactors
  • Enzymatic reactions
  • Inhabitation enzymatic reactions
  • Cell selection and sterilization
  • Cell and cell cluster as bioreactor
  • Diffusion and reaction in cell clusters
  • Effective diffusion coefficient
  • Disposable bioreactors
  • Microreactors
  • Microfluidic and lab on chips
  • Bioreactor for bioprinted tissue
Reading Resources:
Two first items are the main ones and the rest are for detailed study.
  • Elements of chemical reaction engineering. 4th edit, Fogler. Chapters 1,2,3,4, 12, 13
  • Bioreactors for tissue engineering. Principles, design, and operation. J. Chaudhuri and M. Al-Rubeai
  • Bioreactor systems for tissue engineering. C. Kasper …
  • Enzymatic kinetics, a modern approach. A.G. Marangoni
  • Fundamentals of biochemical engineering. R. Dutta
  • Basic bioreactor design. Klass van’t Riet ….
  • Bioprocess engineering principles. P.M. Doran
  • Culture of animal cells (A manual of basic technique). R.I. Freshney
  • Midterm                                   35%
  • Homework                               10%
  • Class project and report           20%
  • Participation in discussion       5%
  • Final                                         30%


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