Course Description:
Protein engineering describes methods of changing the protein sequence to achieve the desired result for a specific application.
Course Goals and Objectives:
The aim of the course is to study the techniques in which modify a protein sequence by substituting, inserting, or deleting nucleotides into an encoding gene, with the goal of obtaining a modified protein that is more suitable than an unmodified protein for a specific application or purpose.
Course Topics:
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Introduction to protein engineering
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Structures and conformations of proteins
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Expression of proteins
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Protein sequencing and purification
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DNA Sequencing
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Natural mutagenesis and recombination
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Vectors
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Recombinant DNA technology and cloning
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Engineering of antibodies, enzymes
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Applications of recombinant proteins in tissue engineering and biomaterials
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Computer methods in protein modeling
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In vivo and in vitro tests for the detection and evaluation of proteins
The course aims to:
- describe the structure and classification of proteins
- describe the methods of protein recombination
- structure of proteins, and relate this information to the function of proteins
- explain how proteins can be used for different industrial and academic purposes
- Explain the applications of recombinant proteins in tissue engineering and biomaterials
- design primers to introduce mutations by means of PCR
Reading Resources:
- T.A., gene Cloning and DNA Analysis: An Introduction, 7th ed, 2016, Wiley-Blackwell.
- Jeremy B., Tymoczko J., Stryer L., Biochemistry. 5th ed., 2006, New York, NY: W.H. Freeman and Company.
- Miller M., Arndt M., Arndtk, Protein Engineering Protocols (Methods in Molecular Biology), 2006, Humana Press.
- Lutz S., Bornscheuer U., Protein Engineering Handbook, 2006, Wiley-VCH.
- Alberghina L., Protein Engineering for Industrial Biotechnology, 2000, CRC.
- Twyman R.M., Principles of Proteomics (Advanced Text Series), 2004, Bias Scientific Publication.
Evaluation
Homework, term projects, midterm and final exam
.