Content
Pepole
CV
(Department) Biomedical Engineering (Division)
(Level and Major)
(Level and Major)
Course Title Engineering of Cell Culture Techniques and Lab
Number of Credits 3 Prerequisite
Course Description:
This course helps student learn cell culture and analysis techniques.
Course Topics:
- An introduction to cell culture in tissue engineering
- History and applications of cell culture
- Cell culture lab equipment
- Biosafety
- Different types of cell culture
- Cell culture medium components
- Cell growth phases
- Cell culture protocols
- Sterilization techniques
- Culture medium preparation
- Thawing frozen cells
- Cell counting techniques
- Cell passaging protocols
- Cell freezing protocols
- Cell fixation methods
Reading Resources:
- R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Wiley, 2010
- Gordana Vunjak-Novakovic, R. Ian Freshney, Culture of Cells for Tissue Engineering, Wiley, 2006
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