(Department)  Biomedical Engineering         (Division)      Biomaterials
 
 (Level and Major)   BSc         
 

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Course Title                Applied Chemistry for Biomaterials Laboratory                              
 
Number of Credits       3             Prerequisite 

Course Description:
This laboratory course is an introduction to the techniques commonly used in the organic and bio chemistry laboratory. Introduces basic laboratory techniques; such as distillation, crystallization, extraction, chromatography, characterization by physical methods, purification, and characterization of products from Microscale syntheses.
 
Course Topics:

1. Safety guideline and survival manual in chemistry laboratory
2. Chemical and biological laboratory glassware (how to use pipet, flasks, condensers, etc.)
3. Chemical laboratory devices (how to use balance, centrifuge, pH meter, etc.)
4. Preparing solutions with exact concentration (calculation and procedure)
5. Recrystallization
6. Melting point
7. Distillation (simple, fractional, rotary evaporator)
8. Extraction from solids and liquids
9. Chromatography (TLC, column)
10. Introduction to laboratory analysis devices (HPLC, GC, UV-Vis spectroscopy, etc.)
11. Alkene synthesis and reaction workup
12. Molecular weight determination
13. Synthesis of Aspirin
14. Synthesis of esters
15. PBS solution preparation
16. Study of enzymatic reactions(titration)

 
The course aims to:

1. Use various laboratory equipment to generate data
2. Demonstrate their critical thinking, scientific and logical reasoning
3. Assemble glassware and perform the following techniques as a part of synthetic procedures: aqueous workup, distillation, reflux, separation, isolation, and crystallization.
4. Utilize mathematical knowledge gained from general chemistry to perform common calculations
5. Write a formal lab report
6. Engage in safe laboratory practices by handling laboratory glassware, equipment, and chemical reagents appropriately, using general guidelines and basic knowledge about the common hazards associated with them in an organic chemistry laboratory

 
Reading Resources:

• Safety in chemistry and biochemistry laboratory, picot and grenouillet
• Organic Chemistry Laboratory Notebook ,Thomson Brooks

 
Evaluation: