Number of Credits 3Prerequisite Same as Tissue Engineering Field
Course Description:
We are going to learn about the third elements for tissue engineering; bioreactor. You would learn how to use different area of science to mimic the microenvironment inside body for cells. This microenvironment includes the geometry of the environment, media for cell culture, the flow of media, and different signaling.
Course Goals and objectives:
At the end of this course, students should be able to:
Identify the key required parameters for their tissue of the interest (biophysics, biology, biochemistry, and biomechanics)
Gather the required data for the important parameters
Propose the initial characters of the required bioreactor
Identify the inputs and outputs
Simplify the bioreactor
Course Topics:
Bioreactor for tissue engineering; where different fields meet each other
A review on the basic concepts and definitions in chemical and biological reactions (reaction, reaction rate, reaction element, open system vs closed one)
Batch reactor
Continuous stirred reactor (CSTR)
Plug flow reactor (PFR) and packed bed reactor (PBR)
Conversion and design equations for batch and CSTR reactors
Conversion and design equations for PFR and PBR
Series reactors vs parallel reactors
Enzymatic reactions
Inhabitation enzymatic reactions
Cell selection and sterilization
Cell and cell cluster as bioreactor
Diffusion and reaction in cell clusters
Effective diffusion coefficient
Disposable bioreactors
Microreactors
Microfluidic and lab on chips
Bioreactor for bioprinted tissue
Reading Resources:
Two first items are the main ones and the rest are for detailed study.
Elements of chemical reaction engineering. 4th edit, Fogler. Chapters 1,2,3,4, 12, 13
Bioreactors for tissue engineering. Principles, design, and operation. J. Chaudhuri and M. Al-Rubeai
Bioreactor systems for tissue engineering. C. Kasper …
Enzymatic kinetics, a modern approach. A.G. Marangoni
Fundamentals of biochemical engineering. R. Dutta
Basic bioreactor design. Klass van’t Riet ….
Bioprocess engineering principles. P.M. Doran
Culture of animal cells (A manual of basic technique). R.I. Freshney
Evaluation:
Midterm 35%
Homework 10%
Class project and report 20%
Participation in discussion 5%
Final 30%
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Topic URL in Department of Biomedical Engineering website: http://aut.ac.ir/find-2.2485.6473.en.html Back to content primary page