(Department)  Biomedical Engineering         (Division)     
 
 (Level and Major)           
 

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Course Title              Engineering of Cell Culture Techniques and Lab                           
 
Number of Credits       3             Prerequisite  

Course Description:
This course helps student learn cell culture and analysis techniques.
 
Course Topics:

• An introduction to cell culture in tissue engineering
• History and applications of cell culture
• Cell culture lab equipment
• Biosafety
• Different types of cell culture
• Cell culture medium components
• Cell growth phases
• Cell culture protocols
• Sterilization techniques
• Culture medium preparation
• Thawing frozen cells
• Cell counting techniques
• Cell passaging protocols
• Cell freezing protocols
• Cell fixation methods

           
Reading Resources:

• R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Wiley, 2010
• Gordana Vunjak-Novakovic, R. Ian Freshney, Culture of Cells for Tissue Engineering, Wiley, 2006

 
Evaluation: